e faecalis atcc 700802  (ATCC)

Journal: BMC Immunology

Article Title: Enterococcus faecalis KU-EF-004 enhances efficacy of anti-CTLA-4 immunotherapy for colorectal cancer via dendritic cell activation and gut microbiota modulation

doi: 10.1186/s12865-026-00827-7

Figure Lengend Snippet: Antitumor effect of E. faecalis ATCC and KU-EF-004 oral administrations and ICI against MC38. Mean tumor growth curves Mice were divided into four groups: PBS, PBS + anti-PD-1 + anti-CTLA-4 (ICI), E. faecalis ATCC + ICI, and KU-EF-004 + ICI ( n = 4 for all groups). The KU-EF-004 + ICI group showed suppressed tumor growth compared to the other groups, including the E. faecalis ATCC group, at day 19, although the difference was not statistically significant. Each data point presents the average tumor volumes of each group (mean, ± SEM). Data are representative of one independent experiment

Article Snippet: KU-EF-004 strains, along with E. faecalis ATCC 700,802 strain, were each cultured and collected by centrifugation.

Techniques:

Journal: Microbiology Spectrum

Article Title: Hidden diversity in Enterococcus faecalis revealed by CRISPR2 screening: eco-evolutionary insights into a novel subspecies

doi: 10.1128/spectrum.01428-25

Figure Lengend Snippet: Species and subspecies delineation based on dDDH thresholds. Phylogenetic trees were inferred using FastME 2.1.6.1 from GBDP distances calculated from genome sequences. Branch lengths are scaled according to the GBDP distance formula d5. The numbers above the branches represent GBDP pseudo-bootstrap support values > 60% from 100 replications. The trees were midpoint-rooted. The taxonomic classifications at the species and subspecies levels are represented by color codes on the right side of each tree, based on the dDDH values obtained and the established thresholds (≥70% dDDH for species and ≥79% dDDH for subspecies) ( A ) Whole-genome sequence-based phylogeny of E. faecalis 209EA1 (representative strain of the genetically related group of E. faecalis lacking CRISPR2) and its closest related type-strains, as determined by TYGS by default. ( B ) Phylogeny focused exclusively on query genomes, encompassing all 16 E. faecalis strains lacking CRISPR2 along with E. faecalis reference strains, including the type strain ATCC 19433. Sequence types (STs) of each strain are shown in parentheses. An asterisk (*) indicates a type strain of a proposed species with nomenclatural status not yet validly published.

Article Snippet: We observed that 20 genes from subspecies A and 20 from subspecies B exhibited significant similarity to the same reference sequences from E. faecalis strain ATCC 700802 /V583 in our BLASTp analysis against UniProtKB.

Techniques: Sequencing

Journal: Microbiology Spectrum

Article Title: Hidden diversity in Enterococcus faecalis revealed by CRISPR2 screening: eco-evolutionary insights into a novel subspecies

doi: 10.1128/spectrum.01428-25

Figure Lengend Snippet: Comprehensive phylogeny of the Enterococcus genus illustrating subspecies-level cladogenesis of E. faecalis . The tree was estimated using IQ-TREE under the LG + F + I + R9 substitution model with 1,000 bootstrap replicates, based on the concatenation and alignment of 751 protein sequences corresponding to single-copy orthologs present in 95.8% of the 117 sampled enterococci and the outgroup strain Vagococcus fluvialis DSM 5731. The tree is presented at different scales. The global phylogeny on the left shows relationships between taxonomic species, with E. faecalis strains collapsed into their respective subspecies clades, which are highlighted by colored regions enclosed in a dashed rectangle. On the right, the E. faecalis clade is detailed at a fine scale, highlighting subspecies clusters (subspecies A in light orange and subspecies B in light teal). Red dots represent type strains; blue dots indicate E. faecalis model strains V583 and OG1RF; the black dot marks the E. faecalis reference genome from the NCBI data sets. Stars denote nodes with bootstrap support <95%.

Article Snippet: We observed that 20 genes from subspecies A and 20 from subspecies B exhibited significant similarity to the same reference sequences from E. faecalis strain ATCC 700802 /V583 in our BLASTp analysis against UniProtKB.

Techniques:

Journal: Microbiology Spectrum

Article Title: Hidden diversity in Enterococcus faecalis revealed by CRISPR2 screening: eco-evolutionary insights into a novel subspecies

doi: 10.1128/spectrum.01428-25

Figure Lengend Snippet: E. faecalis intraspecific phylogeny showcasing the distribution of CRISPR, antimicrobial resistance (AMR), and virulence genes across subspecies A and B. The tree was estimated using IQ-TREE under the GTR + G substitution model with 10,000 bootstrap replicates, based on SNPs extracted from the concatenated alignment of the 1,650 core genes present in all 55 sampled E. faecalis genomes. Rooted at the midpoint, the tree depicts E. faecalis subspecies A and B as the deepest divergences within the species, shown in colored ranges (subspecies A in light orange and subspecies B in light teal). Stars denote nodes with bootstrap support <95%. Strain names are followed by their assigned sequence types (STs) in each leaf label. The colored strip adjacent to the strains indicates their respective isolation sources: human (red), animal (green), coastal water (blue), animal product (orange), beef processing environments (yellow), or unknown origin (gray). Screened genetic traits are shown as binary data in the next panels (filled shapes indicate trait presence and omitted shapes indicate absence), with CRISPR loci as checkmarks, antimicrobial resistance genes as black squares, and virulence genes as dark gray squares.

Article Snippet: We observed that 20 genes from subspecies A and 20 from subspecies B exhibited significant similarity to the same reference sequences from E. faecalis strain ATCC 700802 /V583 in our BLASTp analysis against UniProtKB.

Techniques: CRISPR, Sequencing, Stripping Membranes, Isolation